Mutate DNA

Mutate DNA introduces base changes into a DNA sequence. You can select the number of mutations to introduce, and whether or not to preserve the first and last three bases in the sequence, to reflect selection acting to maintain start and stop codons. The position of each mutation is chosen randomly, and multiple mutations can occur at a single site. Mutated sequences can be used to evaluate the significance of sequence analysis results.

Paste the raw sequence or one or more FASTA sequences into the text area below. Input limit is 100000 characters.

>mRNA /gene=fem-2
atgtctgattcgctaaatcatccatcgagttctacggtgcatgcagatgatggattcgag
ccaccaacatctccggaagacaacaacaaaaaaccgtctttagaacaaattaaacaggaa
agagaagcgttgtttacggatctattcgcagatcgtcgacgaagcgctcgttctgtgatt
gaagaagctttccaaaacgaactcatgagtgctgaaccagtccagccaaacgtgccgaat
ccacattcgattcccattcgtttccgtcatcaaccagttgctggacctgctcatgatgtt
ttcggagacgcggtgcattcaatttttcaaaaaataatgtccagaggagtgaacgcggat
tatagtcattggatgtcatattggatcgcgttgggaatcgacaaaaaaacacaaatgaac
tatcatatgaaaccgttttgcaaagatacttatgcaactgaaggctccttagaagcgaaa
caaacatttactgataaaatcaggtcagctgttgaggaaattatctggaagtccgctgaa
tattgtgatattcttagcgagaagtggacaggaattcatgtgtcggccgaccaactgaaa
ggtcaaagaaataagcaagaagatcgttttgtggcttatccaaatggacaatacatgaat
cgtggacagagtgacatttcacttcttgcggtgttcgatgggcatggcggacacgagtgc
tctcaatatgcagctgctcatttctgggaagcatggtccgatgctcaacatcatcattca
caagatatgaaacttgacgaactcctagaaaaggctctagaaacattggacgaaagaatg
acagtcagaagtgttcgagaatcttggaaaggtggaaccactgctgtctgctgtgctgtt
gatttgaacactaatcaaatcgcatttgcctggcttggagattcaccaggttacatcatg
tcaaacttggagttccgcaaattcactactgaacactccccgtctgacccggaggaatgt
cgacgagtcgaagaagtcggtggccagatttttgtgatcggtggtgagctccgtgtgaat
ggagtactcaacctgacgcgagcactaggagacgtacctggaagaccaatgatatccaac
aaacctgataccttactgaagacgatcgaacctgcggattatcttgttttgttggcctgt
gacgggatttctgacgtcttcaacactagtgatttgtacaatttggttcaggcttttgtc
aatgaatatgacgtagaagattatcacgaacttgcacgctacatttgcaatcaagcagtt
tcagctggaagtgctgacaatgtgacagtagttataggtttcctccgtccaccagaagac
gtttggcgtgtaatgaaaacagactcggatgatgaagagagcgagctcgaggaagaagat
gacaatgaatag

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Introduce mutations into each sequence.
The first three bases in each sequence be mutated.
The last three bases in each sequence be mutated.

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