Buffer Calculator
Buffer:
Maleate (pKa=2.0)
Phosphate (pKa1=2.15)
Chloroacetate (pKa=2.88)
Citrate (pKa1=3.14)
Formate (pKa=3.75)
Succinate (pKa1=4.19)
Benzoate (pKa=4.2)
Acetate (pKa=4.76)
Citrate (pKa2=4.76)
Propionate (pKa=4.86)
Pyridine (pKa=5.23)
Succinate (pKa2=5.57)
Piperazine (pKa2=5.55)
MES (pKa=6.21)
Carbonate (pKa1=6.37)
Citrate (pKa3=6.39)
Bis-tris (pKa=6.46)
ADA (pKa=6.62)
PIPES (pKa=7.1)
ACES (pKa=6.91)
BES (pKa=7.26)
MOPS (pKa=7.31)
Phosphate(pKa2=7.2)
TES (pKa=7.61)
HEPES (pKa=7.66)
Tris (pKa=8.06)
Tricine (pKa=8.26)
Bicine (pKa=8.46)
TAPS (pKa=8.51)
Ethanolamine (pKa=9.5)
CHES (pKa=9.41)
Carbonate (pKa2=10.25)
CAPS (pKa=10.51)
Methylamine (pKa=10.62)
Piperidine (pKa=11.12)
Phosphate (pKa3=12.33)
Volume:
5000
2000
1000
500
200
100
mL
pH:
Concentration:
mM
Ionic Strength:
Ionic Strength is defined if checked
Set the ionic strength to
mM
With:
NaCl
KCl
Na2SO4
Other salt
Temperature:
Use the buffer at
°C
Make the buffer at
°C
Programmed by Dr. Rob Beynon, University of Liverpool, UK