µg DNA
x |
pmol |
x |
106pg |
x |
1 |
x 2 x |
kb |
= pmol DNA |
|
|
|
|
660pg |
1µg |
N |
1000bp |
N is the
number of nucleotides (in kb),
660pg/pmol is the
average molecular weight
of a single nucleotide pair,
2 is the number of ends in a linear DNA
molecule,
and kb/1000bp is a conversion factor from
kilobases to base pairs.
Based on BioMath programs created and
copyrighted by Promega Corporation.